- Product NameRecombinant Murine Mesencephalic Astrocyte-Derived Neurotrophic Factor
- Host SpeciesEscherichia coli.
- Purification> 98 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rMuMANF as determined by LAL method.
- UniprotQ9CXI5
- Calculated MW Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
- Target Sequence LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
