- Product NameRecombinant Murine Growth Differentiation Factor 7/Bone Morphogenetic Protein-12
- Host SpeciesEscherichia coli.
- Purification> 95 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rMuGDF-7/BMP-12 as determined by LAL method.
- UniprotP43029
- Gene ID238057
- Calculated MW Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
- Target Sequence TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
