- Product NameRecombinant Canine Tumor Necrosis Factor-alpha/TNFSF2
- Host SpeciesEscherichia coli.
- Purification> 95 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg in the presence of actinomycin D.
- EndotoxinLess than 1 EU/ug of rCaTNF-α as determined by LAL method.
- UniprotP51742
- Gene ID403922
- Calculated MW Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
- Target Sequence VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
