- Product NameRecombinant Rat Interleukin-1 alpha
- Host SpeciesEscherichia coli.
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 x108 IU/mg.
- EndotoxinLess than 1 EU/ug of rRtIL-1α as determined by LAL method.
- UniprotP16598
- Gene ID24493
- Calculated MW Approximately 17.8 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
- Target Sequence SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
