- Product NameRecombinant Rat Interleukin-1 beta
- Host SpeciesEscherichia coli.
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
- EndotoxinLess than 1 EU/ug of rRtIL-1β as determined by LAL method.
- Alternative Names Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP
- UniprotQ63264
- Calculated MW Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
- Target Sequence MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
