- Product NameRecombinant Rat Interleukin-10
- Host SpeciesEscherichia coli.
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rRtIL-10 as determined by LAL method.
- UniprotP29456
- Gene ID25325
- Calculated MW Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
- Target Sequence SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN
- Formulation Lyophilized from a 0.2 μm filtered solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
