• Product NameRecombinant Rat Fibroblast Growth Factor 18
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x10⁶ IU/mg.
• EndotoxinLess than 1 EU/ug of rRtFGF-18 as determined by LAL method.
• UniprotO88182
• Gene ID29369
• Calculated MW Approximately 21.0 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
• Target Sequence EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Rat FGF-18 is encoded by the FGF18 gene in rats. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18�?�?mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 also has significantroles in lung development and has an anabolic effect on cartilage formation. Additionally, it has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Rat FGF-18 shares 98 % amino acidsequence identity with human.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting