- Product NameRecombinant Rat Platelet-derived Growth Factor-BB
- Host SpeciesEscherichia coli.
- Purification> 98 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg.
- EndotoxinLess than 0.1 EU/ug of rRtPDGF-BB as determined by LAL method.
- UniprotQ05028
- Gene ID24628
- Calculated MW Approximately 24.4 kDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).
- Target Sequence SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 6.9.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
