- Product NameRecombinant Porcine Interleukin-1 Receptor Antagonist Protein
- Host SpeciesEscherichia coli.
- Purification> 97 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x104 IU/mg in the presence of 75 pg/ml rPoIL-1α.
- EndotoxinLess than 0.1 EU/ug of rPoIL-1RA as determined by LAL method.
- UniprotQ29056
- Gene ID397499
- Calculated MW Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
- Target Sequence HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, 1 mM DTT.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
