• Product NameRecombinant Human Growth Regulated Protein-gamma/CXCL3
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
• EndotoxinLess than 1 EU/ug of rHuGRO-γ/CXCL3 as determined by LAL method.
• Alternative Names    GRO-gamma, MIP2-beta
• UniprotP19876
• Gene ID2921
• Calculated MW Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
• Target Sequence ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXCL3, also named GRO-γ, is belonging to the CXC chemokine family and encoded by the gene CXCL3. CXCL3/GRO-γ shares 86 % amino acid sequence with CXCL1/GRO-α. All three human GROs (GRO-α, GRO-β, GRO-γ) are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted by monocytes and macrophages. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three GROs can bind with high affinity to the IL-8 receptor type B.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting