• Product NameRecombinant Human Neutrophil Activating Protein-2/CXCL7
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 1.0-10.0 ng/ml.
• EndotoxinLess than 1 EU/ug of rHuNAP-2/CXCL7 as determined by LAL method.
• Alternative Names    CXCL7, LDGF, MDGF, Small-inducible cytokine B7
• UniprotP02775
• Gene ID5473
• Calculated MW Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
• Target Sequence AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Neutrophil activating protein-2 also named CXCL7 is an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein. It belongs to the CXC chemokine family and is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant human CXCL7 contains 70 amino acids which is a single non-glycosylated polypeptide chain. In addition, The human CXCL7 shares 53 % and 58 % a.a. sequence identity with mouse and rat CXCL7.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting