• Product NameRecombinant Human CXCL16
• Host SpeciesE. coli
• Purification> 97 % by SDS-PAGE analyses.
• Biological ActivityMeasured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6. The ED50 for this effect is 2.5-12 ng/ml.
• EndotoxinLess than 1.0 EU/ug of rHuCXCL16 as determined by LAL method.
• Calculated MW Approximately 10 kDa, a single non-glycosylated polypeptide chain containing 89 amino acids.
• Target Sequence Asn49-Pro137; Accession # NP_071342
• Formulation Lyophilized from 0.2 μm filtered concentrated solution in PBS.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting