• Product NameRecombinant Human Macrophage Inflammatory Protein-1 beta/CCL4
• Host SpeciesE.coli
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.
• EndotoxinLess than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.
• Alternative Names    G-26 T-lymphocyte-secreted protein, HC21, LAG-1, MIP-1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, ACT-2
• UniprotP13236
• Gene ID6351
• Calculated MW Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
• Target Sequence APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
• Formulation Less than 1 EU/ug of rHuMIP-1��/CCL4 as determined by LAL method.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ��C as supplied.- 1 month, 2 to 8 ��C under sterile conditions after reconstitution.- 3 months, -20 to -70 ��C under sterile conditions after reconstitution.

CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with murine and rat CCL4, respectively. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting