• Product NameRecombinant Human Eotaxin-2/CCL24
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.
• EndotoxinLess than 1 EU/ugof rHuEotaxin-2/CCL24 as determined by LAL method.
• Alternative Names    CK-beta-6, MPIF-2, Small-inducible Cytokine A24
• UniprotO00175
• Gene ID6369
• Calculated MW Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.
• Target Sequence VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CCL24 is a secreted protein, encoded by CCL24 gene in humans located on Chr.7, and produced by activated monocytes and T lymphocytes. CCL24 has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting