• Product NameRecombinant Murine Platelet Factor-4/CXCL4
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
• EndotoxinLess than 1 EU/ugof rMuPF-4/CXCL4 as determined by LAL method.
• UniprotQ9Z126
• Gene ID56744
• Calculated MW Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
• Target Sequence VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting