• Product NameRecombinant Murine Monokine Induced by Interferon-gamma/CXCL9
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.
• EndotoxinLess than 1 EU/ug of rMuMIG/CXCL9 as determined by LAL method.
• Alternative Names    Gamma-interferon-induced Monokine, Small-inducible Cytokine B9
• UniprotP18340
• Gene ID17329
• Calculated MW Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 105 amino acids.
• Target Sequence TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-γ. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting