• Product NameRecombinant Murine Exodus-2/CCL21
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.
• EndotoxinLess than 1 EU/ugof rMuExodus-2/CCL21 as determined by LAL method.
• Alternative Names    6Ckine, Beta-chemokine Exodus-2, SLC
• UniprotP86792
• Gene ID65956
• Calculated MW Approximately 12.1 kDa, a single, non-glycosylated polypeptide chain containing 110 amino acids.
• Target Sequence SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CCL21 is encoded by CCL21 gene that is located in Chr. 4 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxylterminal domain. Human and mouse CCL21 are highly conserved, exhibiting 86 % amino acid sequence identity. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting