• Product NameRecombinant Rat Neutrophil Activating Peptide 2/CXCL7
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.1-1.0 ng/ml.
• EndotoxinLess than 1 EU/ug of rRtNAP-2/CXCL7 as determined by LAL method.
• Alternative Names    CXC Chemokine RTCK1, Chemokine (C-X-C Motif) Ligand 7, Isoform CRA_b, Protein Ppbp.
• Calculated MW Approximately 6.8 kDa, a single non-glycosylated polypeptide chain containing 62 amino acids.
• Target Sequence IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CXCL7 is a small cytokine which is also an isoform of Beta-Thromboglobulin or Pro-Platelet basic protein belonging to the CXC chemokine family and it is released in large amounts from platelets following their activation. CXCL7 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. Recombinant Rat CXCL7 contains 62 amino acids which is a single non-glycosylated polypeptide chain. In addition, The rat CXCL7 shares 58 % and 77 % a.a. sequence identity with human and mouse CXCL7.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting