• Product NameRecombinant Rat Fractalkine/CX3CL1
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-10 ng/ml.
• EndotoxinLess than 1 EU/ug of rRtFractalkine/CX3CL1 as determined by LAL method.
• Alternative Names    CX3C Membrane-anchored Chemokine, Neurotactin, Small-inducible Cytokine D1
• UniprotO55145
• Gene ID89808
• Calculated MW Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Rat Fractalkine.
• Target Sequence QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNG
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2x PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CX3CL1 recently identified through bioinformatics is the only known member of the CX3C chemokine family and it is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucinlike talk. The soluble form of CX3CL1 is chemotactic for T-cells and monocytes, but not for neutrophils. In addition, it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. Recombinant Rat CX3CL1 contains 76 amino acids and it shares approximately 86 % and 83 % amino acid sequence homology with the murine and human protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting