• Product NameRecombinant Rat Eotaxin/CCL11
• Host SpeciesEscherichia coli.
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
• EndotoxinLess than 1 EU/ugof rRtEotaxin/CCL11 as determined by LAL method.
• Alternative Names    Small-inducible Cytokine A11
• UniprotP97545
• Gene ID29397
• Calculated MW Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
• Target Sequence HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting