• Product NameRecombinant Rat Eotaxin-2/CCL24
• Host SpeciesEscherichia coli.
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.
• EndotoxinLess than 1 EU/ug of rRtEotaxin-2/CCL24 as determined by LAL method.
• Alternative Names    CK-beta-6, MPIF-2, Small-inducible Cytokine A24
• Calculated MW Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2.
• Target Sequence MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting