• Product NameRecombinant Human Parathyroid Hormone 1-84, 15N Stable Isotope Labeled
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x10⁴ IU/mg.
• EndotoxinLess than 1 EU/ugof rHuPTH1-84, 15N as determined by LAL method.
• Alternative Names    Parathormone, Parathyrin
• UniprotP01270
• Gene ID5741
• Calculated MW Approximately 9551 Da, a single non-glycosylated polypeptide chain containing 84 amino acids. 15N stable isotope labeled.
• Target Sequence SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. PTH is a critical hormone in the regulation of Ca2+ homeostasis. PTH is secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting