• Product NameRecombinant Human Cu/Zn Superoxide Dismutase, His
• Host SpeciesEscherichia coli.
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 1600 U/mg.
• EndotoxinLess than 1 EU/ugof rHuCu/Zn SOD, His as determined by LAL method.
• Alternative Names    SOD1
• UniprotP00441
• Gene ID6647
• Calculated MW Approximately 39.9 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 x 189 amino acids with Met, Gly and 10 x His at N-terminus.
• Target Sequence MGHHHHHHHH HHSSGHIEGR HMTYARAAAR QARALEATKA VCVLKGDGPV QGIINFEQKE SNGPVKVWGS IKGLTEGLHG FHVHEFGDNT AGCTSAGPHF NPLSRKHGGP KDEERHVGDL GNVTADKDGV ADVSIEDSVI SLSGDHCIIG RTLVVHEKAD DLGKGGNEES TKTGNAGSRL ACGVIGIAQ
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Superoxide dismutase catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. Cu/Zn superoxide dismutase also named as SOD1, is an enzyme encoded by the SOD1 gene in humans, located on chromosome 21. The SOD1 binds Cu and Zn ions and is one of three SODs responsible for destroying free superoxide radicals in the body. It has been shown to interact with CCS and Bcl-2. The malfunction of SOD1 may increase the risk of illnesses like age-related muscle mass loss (sarcopenia), early development of cataracts, macular degeneration, thymic involution, hepatocellular carcinoma, shortened lifespan, keratoconus and amyotrophic lateral sclerosis.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting