- Product NameRecombinant Human TP53-induced Glycolysis and Apoptosis Regulator-TAT
- Host SpeciesEscherichia coli.
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
- EndotoxinLess than 0.1 EU/ugof rHuTIGAR-TAT as determined by LAL method.
- UniprotQ9NQ88
- Gene ID57103
- Calculated MW Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids.
- Target Sequence MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
