• Product NameRecombinant Human Otoraplin
• Host SpeciesEscherichia coli.
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityData Not Available.
• EndotoxinLess than 1 EU/ug of rHuOTOR as determined by LAL method.
• Alternative Names    Fibrocyte-derived Protein, Melanoma Inhibitory Activity-like Protein
• Calculated MW Approximately 12.7 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids.
• Target Sequence VHGIFMDRLA SKKLCADDEC VYTISLASAQ EDYNAPDCRF INVKKGQQIY VYSKLVKENG AGEFWAGSVY GDGQDEMGVV GYFPRNLVKE QRVYQEATKE VPTTDIDFFC E
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150mM NaCl.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

OTOR also named Otoraplin and MIAL, is a 15 kDa disulfide bonded homodimer, which is secreted via the Golgi apparatus and is a member of the MIA/OTOR family. Members of this family which also includes MIA, MIA2, and TANGO share a Src homology-3 (SH3)-like domain. OTOR is mainly expressed in the cochlea of the inner-ear and appears to be involved in early chondrogenesis of the otic capsule, which is required for normal inner ear development and auditory function. The OTOR shares high sequence identity with the mouse (90%), chicken (80%), and bullfrog (60%) orthologs and with the related human CDRAP/MIA protein (43%).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting