• Product NameRecombinant Human Osteopontin/OPN
• Host SpeciesMouse myeloma cell line, NS0
• Purification> 95 % by SDS-PAGE analyses.
• Biological ActivityMeasured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x10⁵ cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
• EndotoxinLess than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.
• Calculated MW Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.
• Target Sequence Ile17-Asn300, with a C-terminal 6-His tag; Accession # NP_000573.1
• Formulation Lyophilized from 0.2 μm filtered concentrated solution in PBS, pH 7.2.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Osteopontin (OPN), also known as SPP1, Eta-1, or BSP, is a highly acidic, 45-75 kDa secreted molecule in the SIBLING family of matricellular proteins. It can be variably modified with O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN binds several integrins and can bind additional integrins after it is proteolytically cleaved. OPN is prominently expressed in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adhesion, and cell migration. OPN expression is upregulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and it contributes to the pathophysiology of these conditions.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting