• Product NameRecombinant Murine Cardiotrophin-1
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 �� 10⁶ IU/mg.
• EndotoxinLess than 1 EU/ug of rMuCT-1 as determined by LAL method.
• UniprotQ60753
• Gene ID13019
• Calculated MW Approximately 21.5 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.
• Target Sequence MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA
• Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ��C as supplied.- 1 month, 2 to 8 ��C under sterile conditions after reconstitution.- 3 months, -20 to -70 ��C under sterile conditions after reconstitution.

Cardiotrophin­1 (CT­1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT­1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT­1 share 80 % a.a. sequence identity and exhibit cross-species activity.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting