- Product NameRecombinant Murine Desert Hedgehog Homolog Cys23IleIle
- Host SpeciesEscherichia coli.
- Purification> 98 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20 μg/ml.
- EndotoxinLess than 0.1 EU/ugof rMuDHH C23II as determined by LAL method.
- UniprotQ61488
- Gene ID13363
- Calculated MW Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids.
- Target Sequence IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
- Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
