• Product NameRecombinant Exendin-4
• Host SpeciesEscherichia coli
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological Activity1. Regulates Glucose levels rapidly;2. Reduces Insulin resistance;3. Reduces Glucagon;4. Reduces HbA1c;5. Stimulates beta cell growth which stimulates insulin production.
• EndotoxinLess than 10 EU/mg of rExendin-4 as determined by LAL method.
• Alternative Names    Exenatide
• UniprotP26349
• Calculated MW Approximately 4.2 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids.
• Target Sequence HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS
• Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppresses inappropriately elevated glucagon secretion, and slows gastric emptying in vivo. It also promotes ß-cell proliferation and neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coli expression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting