• Product NameRNF20 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF IP FC
• Species ReactivityHuman Mouse
• SpecificityRNF20 Antibody detects endogenous levels of total RNF20
• Immunogen DescriptionA synthesized peptide derived from human RNF20
• Alternative Names    BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20;
• Accession No. Uniprot:Q5VTR2
• UniprotQ5VTR2
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• RNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes.

RNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes.

• Western blot analysis of RNF20 expression in (1) 293 cell lysate; (2) HeLa cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting