• Product NameSmad3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificitySmad3 Antibody detects endogenous levels of total Smad3
• Immunogen DescriptionA synthesized peptide derived from human Smad3
• Alternative Names    JV15-2; MADH3; Mad3; Smad 3;
• Accession No. Uniprot:P84022
• UniprotP84022
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 IHC 1:100~1:500 ICC/IF 1:100~1:500 FC 1:50

• Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

• Western blot analysis of Smad3 expression in (1) Jurkat cell lysate; (2) Rat liver lysate.
• Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody.
• Immunofluorescent analysis of Hela cells, using Smad3 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting