• Product NameSmad4 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificitySmad4 Antibody detects endogenous levels of total Smad4
• Immunogen DescriptionA synthesized peptide derived from human Smad4
• Alternative Names    MAD homolog 4; MAD, mothers against decapentaplegic homolog 4; MADH4; Mothers against decapentaplegic homolog 4; DPC4; hSMAD4; JIP;
• Accession No. Uniprot:Q13485
• UniprotQ13485
• Calculated MW 65 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200

• Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

• Western blot analysis of SMAD4 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast cancer, using Smad4 Antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting