• Product Nameactive Caspase-3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman
• Specificityactive Caspase-3 Antibody detects endogenous levels of total active Caspase-3
• Immunogen DescriptionA synthesized peptide derived from human active Caspase-3
• Alternative Names    Apopain precursor; Cysteine protease CPP32; ICE3; CASP-3; CPP32; Caspase-3; SCA-1;
• Accession No. Uniprot:P42574
• UniprotP42574
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100

• Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

• Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.
• Immunofluorescent analysis of HepG2 cells, using active Caspase-3 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting