• Product NameER alpha Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF ChIP FC
• Species ReactivityHuman Mouse Rat Dog
• SpecificityER alpha Antibody detects endogenous levels of total ER alpha
• Immunogen DescriptionA synthesized peptide derived from human ER alpha
• Alternative Names    ER; ESR; ESR1; Era; ESRA; NR3A1;
• Accession No. Uniprot:P03372
• UniprotP03372
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 ChIP 5 ?g/30 ?g FC 1:30

• ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.

• Western blot analysis of ER alpha expression in (1) MCF7 cell lysate; (2)T47-D cell lysate.
• Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody.
• Immunofluorescent analysis of MCF7 cells, using ER alpha Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting