• Product NameMutant p53 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF IP FC
• Species ReactivityHuman
• SpecificityMutant p53 Antibody detects endogenous levels of total Mutant p53
• Immunogen DescriptionA synthesized peptide derived from human Mutant p53
• Alternative Names    Antigen NY-CO-13; TP53; Tumor suppressor p53; Cellular tumor antigen p53; Phosphoprotein p53;
• Accession No. Uniprot:P04637
• UniprotP04637
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50

• Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

• Western blot analysis of Mutant p53 in (1) A431 cell lysate; (2) HEK293 cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting