• Product NameBax Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC IP FC
• Species ReactivityHuman Mouse Rat Hamster
• SpecificityBax Antibody detects endogenous levels of total Bax
• Immunogen DescriptionA synthesized peptide derived from human Bax
• Alternative Names    Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4
• Accession No. Uniprot:Q07812
• UniprotQ07812
• Concentration 0.3mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200
  IP 1:20-1:50
  FC 1:20-1:100

• Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells. -cancer science
• Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting