• Product NameHMGB1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityHMGB1 Antibody detects endogenous levels of total HMGB1
• Immunogen DescriptionA synthesized peptide derived from human HMGB1
• Alternative Names    HMGB1; HMG3; HMG1; SBP 1; Amphoterin;
• Accession No. Uniprot:P09429
• UniprotP09429
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 FC 1:30

• High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.

High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFκB family, ATF-2 and c-Jun to activate transcription.

• Western blot analysis of HMGB1 expression in (1)HeLa cell lysate; (2)Rat brain lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody.
• Immunofluorescent analysis of SW480 cells, using HMGB1 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting