• Product NameHIF-2 alpha Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityHIF-2-alpha Antibody detects endogenous levels of total HIF-2-alpha
• Immunogen DescriptionA synthesized peptide derived from human HIF-2 alpha
• Alternative Names    bHLHe73;ECYT4;EPAS 1;EPAS1;HIF2A;HLF;MOP 2;PASD2
• Accession No. Uniprot:Q99814
• UniprotQ99814
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:50

• Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.

• Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting