• Product NameYY1 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP
• Species ReactivityHuman Mouse Rat
• SpecificityYY1 Antibody detects endogenous levels of total YY1
• Immunogen DescriptionA synthesized peptide derived from human YY1
• Alternative Names    YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1
• Accession No. Uniprot:P25490
• UniprotP25490
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:3000 IHC 1:50~1:200 ICC/IF 1:100~1:500 IP 1:50~1:100

• Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

• Western blot analysis of YY1 expression in (1) HeLa cell lysate; (2) Daudi cell lysate.
• Immunohistochemical analysis of paraffin-embedded human bladder using YY1 Antibody.
• Immunofluorescent analysis of Hela cells, using YY1 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting