• Product NameHistone H3 (acetyl K56) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityHistone H3 (acetyl K56) Antibody detects endogenous levels of total Histone H3 (acetyl K56)
• Immunogen DescriptionA synthesized peptide derived from human Histone H3 (acetyl K56)
• Alternative Names    H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1;
• Accession No. Uniprot:P68431
• UniprotP68431
• Calculated MW 15kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

• Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot analysis of Histone H3 (acetyl K56) expression in C6 cell lysate, treated with TSA.
• Immunohistochemical analysis of paraffin-embedded human colon, using Histone H3 (acetyl K56) Antibody.
• Immunofluorescent analysis of Hela cells, using Histone H3 (acetyl K56) Antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting