• Product NameMCM2 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityMCM2 Antibody detects endogenous levels of total MCM2
• Immunogen DescriptionA synthesized peptide derived from human MCM2
• Alternative Names    BM28; CCNL1; cdc19; CDCL1; cell devision cycle-like 1; cyclin-like 1; D3S3194; MCM2; MCM2 minichromosome maintenance deficient 2, mitotin;
• Accession No. Uniprot:P49736
• UniprotP49736
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200

• The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is also a key component of the pre-replication complex (pre-RC). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication.

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork. This complex is also a key component of the pre-replication complex (pre-RC). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication.

• Western blot analysis of MCM2 expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse colon, using MCM2 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting