• Product NameSmad5 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificitySmad5 Antibody detects endogenous levels of total Smad5
• Immunogen DescriptionA synthesized peptide derived from human Smad5
• Alternative Names    Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5;
• Accession No. Uniprot:Q99717
• UniprotQ99717
• Calculated MW 52kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

• Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad5 is a receptor-regulated Smad (R-Smad). Smad5 is required for normal development of the cardiovascular system in vivo; lack of the Smad5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of Smad5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad5 is a receptor-regulated Smad (R-Smad). Smad5 is required for normal development of the cardiovascular system in vivo; lack of the Smad5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of Smad5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

• Western blot analysis of Smad5 expression in HEK293 cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting