• Product NameNGF Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF
• Species ReactivityHuman Mouse Rat
• SpecificityNGF Antibody detects endogenous levels of total NGF
• Immunogen DescriptionA synthesized peptide derived from human NGF
• Alternative Names    NGF;Beta-NGF;HSAN5;MGC161426;MGC161428;NGFB;proNGF;
• Accession No. Uniprot:P01138
• UniprotP01138
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

• Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation. Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family.

Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation. Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family.

• Western blot analysis of NGF expression in (1)Mouse thyroid lysate;(2) HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded human kidney, using NGF Antibody.
• Immunofluorescent analysis of SH-SY5Y cells, using NGF Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting