• Product NameCD86 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.4C7
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman,Mouse,Rat
• SpecificityCD86 Antibody detects endogenous levels of total CD86
• Immunogen DescriptionA synthesized peptide derived from human CD86
• Target NameCD86
• Alternative Names    CD86;B7-2;B70;CD28LG2;LAB72;MGC34413; CTLA4 counter receptor B72; CLS1; Ly58; MB7;
• Accession No. Uniprot:P42081
• UniprotP42081
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

• Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

• All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:Mouse heart.Lane2:Mouse spleen.Lane3:Mouse lung.Lane4:Rat heart.Lane5:Rat liver.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
• All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:HeLa.Lane2:Jurkat.Lane3:MCF-7.Lane4:SH-SY5Y.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
• All Lanes:CD86 Antibody at 1/ 1000 dilution.Lane1:Raji cell lysate.Proteins at 20ug per laneSecondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 70kDaObserved band size: 70kDa
• Immunofluorescent analysis using the Antibody at 1:50 dilution.4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
• Immunofluorescent analysis of K562 cells, using CD86 Antibody .4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.
• Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody .Tris-EDTA Buffer (pH9.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting