• Product NameB Raf Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.8A9
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityB Raf Antibody detects endogenous levels of total B Raf
• Immunogen DescriptionA synthesized peptide derived from human B Raf
• Target NameBRAF
• Alternative Names    B-RAF; p94; NS7; B-Raf proto-oncogene serine/threonine-protein kinase;BRAF;BRAF1;
• Accession No. Uniprot:P15056
• UniprotP15056
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50

• Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

• All Lanes:B Raf Antibody at 1/ 2000 dilution.Lane1:Mouse brain.Lane2:Rat brain.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 85kDaObserved band size: 85kDa
• All Lanes:B Raf Antibody at 1/ 2000 dilution.Lane1:Human brain lysate.Lane2:HepG2 cell lysate.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 85kDaObserved band size: 85kDa
• Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody.Tris-EDTA Buffer (pH9.0).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting