• Product NameHsc70 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityHsc70 Antibody detects endogenous levels of total Hsc70
• Immunogen DescriptionA synthesized peptide derived from human Hsc70
• Alternative Names    Heat shock cognate 71 kDa protein; HS7C; HSP73; HSP7C; HSPA10; HSPA8;
• Accession No. Uniprot:P11142
• UniprotP11142
• Calculated MW 70kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20 FC 1:30

• Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

• Western blot analysis of Calreticulin expression in (1) HeLa cell lysate; (2) C6 cell lysate.
• Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsc70 Antibody.
• Immunofluorescent analysis of MCF7 cells, using Hsc70 Antibody .
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting