• Product NamePRMT5 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF FC
• Species ReactivityHuman Mouse Rat
• SpecificityPRMT5 Antibody detects endogenous levels of total PRMT5
• Immunogen DescriptionA synthesized peptide derived from human PRMT5
• Alternative Names    HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5; Skb1Hs Methyltransferase;
• Accession No. Uniprot:O14744
• UniprotO14744
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

• Involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK).

Involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p. An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK).

• Western blot analysis of PRMT5 expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded human testis, using PRMT5 Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting