• Product NameHistone H3 (mono methyl K36) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF
• Species ReactivityHuman Mouse
• SpecificityHistone H3 (mono methyl K36) Antibody detects endogenous levels of total Histone H3 (mono methyl K36)
• Immunogen DescriptionA synthesized peptide derived from human Histone H3 (mono methyl K36)
• Alternative Names    H3 histone; HIST1H3A; Histone cluster 1, H3a; H3K36me1
• Accession No. Uniprot:P68431
• UniprotP68431
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 ICC/IF 1:50~1:200

• Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of Histone H3 (mono methyl K36) expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting