• Product NameHistone H4 (tri methyl K20) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificityHistone H4 (tri methyl K20) Antibody detects endogenous levels of total Histone H4 (tri methyl K20)
• Immunogen DescriptionA synthesized peptide derived from human Histone H4 (tri methyl K20)
• Alternative Names    H4F2; HIST; HIST1H4A; Histone H4; H4K20me3;
• Accession No. Uniprot:P62805
• UniprotP62805
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200

• Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot analysis of Histone H4 (tri methyl K20) expression in HeLa cell lysate.
• Immunohistochemical analysis of paraffin-embedded human stomach, using Histone H4 (tri methyl K20) Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting