• Product NameMAP1LC3A Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC ICC/IF IP FC
• Species ReactivityHuman Mouse Rat
• SpecificityMAP1LC3A Antibody detects endogenous levels of total MAP1LC3A
• Immunogen DescriptionA synthesized peptide derived from human MAP1LC3A
• Alternative Names    MAP1LC3;LC3;LC3A;MAP1ALC3;MAP1BLC3 ;MAP1LC3A;
• Accession No. Uniprot:Q9H492
• UniprotQ9H492
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

• Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

• Western blot analysis of MAP1LC3A expression in Hela cell lysate.
• Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody.
• Immunofluorescent analysis of Hela cells, using MAP1LC3A Antibody .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting